Recombinant:Article Title: The Role for Dickkopf-Homolog-1 in the Pathogenesis of Crohn’s Disease-Associated Fistulae
Article Snippet: .. After starvation, HT-29 cells were basolaterally stimulated with either 100 ng/ml human recombinant Interleukin-13 (rhIL-13, R&D), 100 μg/ml of an anti-IL-13 antibody (provided by Novartis AG, Basel, Switzerland), 50 ng/ml human recombinant tumor necrosis factor alpha (TNF-α, Calbiochem/Merck, VWR), 50 ng/ml human recombinant tumor growth factor beta (TGF-β1, Calbiochem/Merck, VWR), 100 ng/ml muramyl dipeptide (MDP, InvivoGen) in a dimethyl sulfoxide (DMSO) – FuGene transfection reagent (Promega) solution, 100 ng/ml ultra-pure lipopolysaccharide (LPS, from E. coli, InvivoGen) and/or 24 μg/ml of an anti-TNF-antibody. .. After starvation, HT-29 cells were basolaterally stimulated with either 100 ng/ml human recombinant Interleukin-13 (rhIL-13, R&D), 100 μg/ml of an anti-IL-13 antibody (provided by Novartis AG, Basel, Switzerland), 50 ng/ml human recombinant tumor necrosis factor alpha (TNF-α, Calbiochem/Merck, VWR), 50 ng/ml human recombinant tumor growth factor beta (TGF-β1, Calbiochem/Merck, VWR), 100 ng/ml muramyl dipeptide (MDP, InvivoGen) in a dimethyl sulfoxide (DMSO) – FuGene transfection reagent (Promega) solution, 100 ng/ml ultra-pure lipopolysaccharide (LPS, from E. coli, InvivoGen) and/or 24 μg/ml of an anti-TNF-antibody.
Transfection:Article Title: The Role for Dickkopf-Homolog-1 in the Pathogenesis of Crohn’s Disease-Associated Fistulae
Article Snippet: .. After starvation, HT-29 cells were basolaterally stimulated with either 100 ng/ml human recombinant Interleukin-13 (rhIL-13, R&D), 100 μg/ml of an anti-IL-13 antibody (provided by Novartis AG, Basel, Switzerland), 50 ng/ml human recombinant tumor necrosis factor alpha (TNF-α, Calbiochem/Merck, VWR), 50 ng/ml human recombinant tumor growth factor beta (TGF-β1, Calbiochem/Merck, VWR), 100 ng/ml muramyl dipeptide (MDP, InvivoGen) in a dimethyl sulfoxide (DMSO) – FuGene transfection reagent (Promega) solution, 100 ng/ml ultra-pure lipopolysaccharide (LPS, from E. coli, InvivoGen) and/or 24 μg/ml of an anti-TNF-antibody. .. After starvation, HT-29 cells were basolaterally stimulated with either 100 ng/ml human recombinant Interleukin-13 (rhIL-13, R&D), 100 μg/ml of an anti-IL-13 antibody (provided by Novartis AG, Basel, Switzerland), 50 ng/ml human recombinant tumor necrosis factor alpha (TNF-α, Calbiochem/Merck, VWR), 50 ng/ml human recombinant tumor growth factor beta (TGF-β1, Calbiochem/Merck, VWR), 100 ng/ml muramyl dipeptide (MDP, InvivoGen) in a dimethyl sulfoxide (DMSO) – FuGene transfection reagent (Promega) solution, 100 ng/ml ultra-pure lipopolysaccharide (LPS, from E. coli, InvivoGen) and/or 24 μg/ml of an anti-TNF-antibody.
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